Macromolecular Crystallography
Shared Resource Director: Alan
Friedman
E-mail: afried@purdue.edu
Facility Director: T. J. Schmidt
Department: Biological Sciences
Phone: (765) 494-5911
Building/Room: LILY B-102B, B-141, B-403
General Information and Services
The essential service of the Resource is to provide
facilities for macromolecular crystallography anticipating direct,
hands-on use by Cancer Center researchers. The facilities include
instruments required for X ray diffraction experiments and for the
crystallization of macromolecules. To achieve this service, experienced
technical staff maintains the facilities, and a resource director
provides scientific guidance in the development of materials and the
use of the instruments.
The crystal growth facilities include four walk-in rooms designed
to provide low-vibration, temperature-controlled environments. Three
rooms with floor areas of roughly 140, 80, and 110 ft2 support fixed
temperatures of 20, 12, and 4°C (0.5°C). The fourth and smallest
room (50 ft2) is intended for crystallization experiments in the presence
of ice-inhibiting agents at a temperature of -5°C.
Three free-standing incubators are available for experiments at other
temperatures.
All rooms and incubators are monitored by a digital scanning thermometer
with a software interface to web-based software that logs and displays
temperature data, and provides warning messages by e-mail. Good to
excellent light microscopes are present in each of the 20, 12, and
4° rooms; those in the 20° room are equipped with digital
camera/video systems.
The diffraction laboratories house X ray generators, diffraction instruments,
and ancillary equipment required for the measurement of single crystal
diffraction patterns. A wide variety of shared and private computational
resources are available to support the analysis, management, and preservation
of the diffraction data. All computational resources and the diffraction
instruments are accessible through the campus network.
X-ray Crystallography Rates
(Rates for Cancer Center members will be discounted)
Diffractometers: $133.00/day
Siemens Instrument: $35.00/day
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